AFLPs: genetic markers for paternity studies in newts (Triturus vulgaris)
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Short Notes AFLPs: genetic markers for paternity studies in newts (Triturus vulgaris)
DNA-based genetic markers can reveal paternity whenever the direct assignment of fathers to offspring is precluded by multiple matings and internal fertilisation (e.g. Hughes, 1998). Microsatellites are the current marker of choice in many behavioural studies, and have revealed important insights into genetic mating systems of European amphibians (e.g., Garner & Schmidt, 2003). However, the num...
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Amplified fragment length polymorphisms are a PCR-based genetic tool used to mark the presence or absence of alleles in a DNA sample. This tool has typically been used for genetic analysis in plants, but research is now being done to see if it is a reliable source for paternity analysis in animals. Samples of Orconectes limosus were collected from Quinebaug River during a previous study and mat...
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Next-generation sequencing is a fast and cost-effective way to obtain sequence data for nonmodel organisms for many markers and for many individuals. We describe a protocol through which we obtain orthologous markers for the crested newts (Amphibia: Salamandridae: Triturus), suitable for analysis of interspecific hybridization. We use transcriptome data of a single Triturus species and design 9...
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ژورنال
عنوان ژورنال: Amphibia-Reptilia
سال: 2006
ISSN: 0173-5373,1568-5381
DOI: 10.1163/156853806776052029